Myelin Repair and Neuroprotection in Multiple Sclerosis by Ian D. Duncan & Robin J. M. Franklin

Author:Ian D. Duncan & Robin J. M. Franklin
Language: eng
Format: epub
Publisher: Springer US, Boston, MA

Fig. 6.5BC directed differentiation towards a peripheral or central myelin-forming cell. When grafted in the demyelinated lesion of the spinal cord, the majority of BC give rise to SC (a–d). Four weeks post grafting, there is a clear colocalization of P0 positive myelin (red b, c) with YFP+ cells (green a, c). Bluo-gal precipitates reveal YFP+ BC (white arrows, d). BC progeny presents a classical morphology of PNS myelin with SC associated in a 1:1 relationship with the axon (d). Higher magnification illustrates the compaction of the newly formed PNS myelin and its basal membrane (black arrow, insert, d). When grafted in the newborn shiverer brain, BC adopt an oligodendroglial fate (e–i). YFP+ cells (green, e, g) expressing MBP (red, f, g) and have features of myelin-forming oligodendrocytes. Insets (e, g) are enlarged views of dotted square illustrating discrete co-expression of MBP and YFP in processes. Ultrastructural analysis confirms the presence of compact donor-derived central myelin (i, inset). Illustration of GFP+ oligodendrocytes in corresponding floating sections prior to processing for electron microscopy (h)

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